The products of snupe for 2 y - snp loci were qualified and quantified with the ion - pair reversed - phase hplc 應(yīng)用建立的hplc方法檢測(cè)單堿基引物延伸反應(yīng)的產(chǎn)物,并且對(duì)產(chǎn)物進(jìn)行了定量。
Then formation of three disulfide binds of hw - ma and rgd - hw was monitored by reversed - phase hplc using air oxidization or reduced and oxidized glutathione respectively . the synthetic peptide was purified by double reversed - phase hplc 用hplc分離純化,得到的合成產(chǎn)物經(jīng)質(zhì)譜鑒定, hw一ma和rgd一hw粗膚的實(shí)際測(cè)定值和復(fù)性后突變體理論值都一致,證明合成和氧化復(fù)性是成功的。
Utilizing ion - pair reversed - phase hplc , four kinds of oligonucleotides with size 18 , 19 , 20 and 21nt , respectively , were separated successfully . furthermore , two kinds of oligonucleotides with same size and difference sequence were successfully separated . linear regression equations of two standard samples have been set up 在普通的高效液相色譜儀上,應(yīng)用反相離子對(duì)高效液相色譜法成功地分離了4種長(zhǎng)度不同的單鏈寡聚核昔酸( 18 、 19 、 20 、 zlnt )以及兩種序列不同的19nt單鏈寡聚核昔酸。